Figure 3.
Distribution of predicted association free energies with SGPA (in blue) of all possible Kazal sequences subject to restrictions stated in the text. Predicted free energies of association of all known subject to restriction ovomucoid third domains with SGPA are shown in red. A white bar indicates the 103 M−1 to 1013 M−1 Ka range of direct measurements. Black arrow marks ΔG°min, the strongest possible association with SGPA, Ka = 8.0 × 1015 M−1. Similar sets of data were obtained for the other five enzymes. They are excluded for brevity.