Table 4.

Kazal sequences predicted to produce the greatest possible Kas

	Sequences	Predicted Ka, M−1
		CHYM	PPE	CARL	SGPA	SGPB	HLE
	P6   P1           P14′P18′
CHYM	QCWCTYEYR… . AN..P	4.0  × 1017	2.3  × 104	1.3  × 109	6.0  × 1011	1.1  × 1010	3.2  × 106
PPE	TEYCTAEYM… . PN..V	3.1  × 106	5.1  × 1013	2.0  × 1010	1.3  × 1012	8.4  × 1010	1.5  × 1010
CARL	WDFCYCEWD… . GN..Q	3.9  × 103	8.6  × 106	1.2  × 1017	5.6  × 109	9.6  × 108	1.7  × 107
SGPA	SYYCTCEYT… . GN..V	4.4  × 108	1.3  × 1011	9.7  × 1010	8.0  × 1015	9.8  × 1013	1.3  × 1010
SGPB	SDYCTCIYK… . GN..V	3.2  × 108	9.8  × 109	1.8  × 1013	3.7  × 1014	2.8  × 1015	6.0  × 108
HLE	RLWCTIEYF… . GN..D	4.6  × 108	7.7  × 109	1.9  × 106	5.9  × 109	2.4  × 108	2.4  × 1016

Based on our SRA, these sequences in a Kazal inhibitor scaffold are predicted to produce greatest possible K_as (in bold) for each enzyme at pH 8.3, 21°C. Only the residues in the consensus contact region (see Fig. 2) are shown. C denotes half cystine, C denotes cysteine.