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Recombinant Maraba virus variants. (a) Based on the amino acid sequence of their L protein, Maraba and Carajas are characterized as species in the genus: vesiculovirus. Line lengths are proportional to genetic distance. (b) Illustration of the Maraba genome outlining the various mutation sites for our single mutants (L123W, V221Y, Q242R) and double mutant (Q242R L123W). (c) One-step growth curves were used to characterize viral productivity of our engineered Maraba mutants.
