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. Author manuscript; available in PMC: 2010 Aug 24.
Published in final edited form as: Cell Physiol Biochem. 2008 Jul 25;22(1-4):57–68. doi: 10.1159/000149783

Figure 2. RT-PCR analysis of recombinant CFTR expression in the complemented CFBE41o− clones.

Figure 2

A. Expression of wtCFTR was prominent in the two stable cell clones c7-6.2wt and c10-6.2wt. B. Using allele-specific primer to detect the expression of the recombinant ΔF508 construct showed prominent expression in clone c4-4.7ΔF, but not in the other clones. C&D. Positive and negative controls are the expression of β-actin and processing the sample without reverse transcriptase (−RT), respectively. The primer pair for wtCFTR amplification was CF7C/CF17; expression of recombinant ΔF508CFTR was detected by primer pair CF81C2/CF17 specific for the TTT deletion in the construct (see Table 1 for sequences).