Table 1. PCR Primers.

Legend: Primers used in this study are shown with their orientation, sequence, and localization. There were 3 different allele-specific reverse primers were used to detect the mRNA specific for wt or ΔF508CFTR. The CF8C primer is specific to the endogenous CTT deletion of Δ508 CFTR, while the CF81C2 primer is specific to the TTT deletion of the recombinant ΔF508CFTR construct. r = reverse; f = forward.

Primer	Orientation	Sequence (5'>3')	CFTR gene localization
CF7C	r	ATAGGAAACACCAAAGATGA	exon 10
CF8C	r	ATAGGAAACACCAATGATAT	exon 10
CF81C2	r	ATTCATCA TAGGAAACACCGATA	exon 10
CF17	f	GAGGGATTTGGGGAATTATTTG	exon 9
HQCF3	f	GACAGTTGTTGGCGGTTGCT	exon 9
HQCF4	r	ACCCTCTGAAGGCTCCAGTTC	exon 10
HGAPDH-R	r	GAAGATGGTGATGGGATTTC
HGAPDH-F	f	GAAGGTGAAGGTAGGAGTC