Fig. 2.
Prevalence of visinin+ cells in RPE cell cultures subjected to reprogramming by various factors/genes. A-F: Representative images of immunodetection of visinin in E6 RPE cell cultures infected with retrovirus RCAS expressing GFP (A; a negative control), ath5 (B), neuroD (C), ngn2 (D), ngn3 (E), and ngn1 (F). G,H: Immunostaining for visinin in an E15 RPE cell culture infected with RCAS-ngn2 (G) or RCAS-ngn1 (H). I: Calculated numbers of visinin+ cells per 242 mm2 area in E6 RPE cell cultures reprogrammed with the different genes as shown. J: Calculated percentage of visinin+ cells in E6 RPE cell cultures reprogrammed with the different genes as shown against ngn1-reprogrmaed culture. Scale bars: 100 μm.