Figure 1.

TNFα treatment induced apoptosis in all four RAS-variant cell lines, with the highest levels observed in DLD-1 and DKs8-kdN cells. A, Overview of RAS variant genotypes – full details can be found in Materials and Methods. B, Cells were stained for cleaved caspase-3 and cleaved PARP and analyzed by flow cytometry for double positive (apoptotic) cells. Different letters indicate significant differences between TNFα-treated RAS-variants at 48 hours, p<0.05.