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. Author manuscript; available in PMC: 2010 Aug 24.
Published in final edited form as: J Virol Methods. 2008 Mar 4;149(1):69–75. doi: 10.1016/j.jviromet.2008.01.005

Figure 1. Polymorphisms in the primer binding site for the ASPCR assay at the 103 position of reverse transcriptase.

Figure 1

These polymorphisms reflect the difference seen in 18 HIV-1 clinical samples from Botswana. Group I matches the published primers exactly (the intentional mismatch at the 2nd position of the primer is done to enhance specificity). The asterisk represents the third nucleotide at the 103 position of reverse transcriptase that confers a drug resistance mutation as the amino acid changes from a lysine (AAA) to an asparagine (AAC or AAT).