Table 2.

Results obtained when polymorphism specific primers used in ASPCR for each of the different groups of binding site polymorphisms (shown in Figure 1). Each group tested had 0% (A), 1% (B), and 5% (C) mutation at position 103 of HIV-1 reverse transcriptase (both AAC and AAT mutations.

A. Plasmids with 100% wt (AAA) at position 103. Results of ASPCR using polymorphism specific primers.
Allele	Percent	I	II	III	IV	V	VI	VII	VIII
103K AAA	(100%)	99.85	99.94	99.87	99.77	99.81	99.51	99.98	99.92
103N AAC	(0%)	0.05	0.01	0.05	0.18	0.05	0.02	0.01	0.004
103N AAT	(0%)	0.002	0.002	0.02	0.011	0.04	0.001	0.001	0.005
103K AAG	(0%)	0.11	0.05	0.07	0.04	0.10	0.47	0.01	0.07
B. Plasmids with 98% wt (AAA), 1% 103N (AAC) and 1% 103N (AAT) at position 103. Results of ASPCR using polymorphism specific primers
Allele	Percent	I	II	III	IV	V	VI	VII	VIII
103K AAA	(98%)	97.53	97.50	97.49	97.68	97.12	97.19	97.29	97.52
103N AAC	(1%)	1.06	1.17	1.24	1.15	1.34	1.36	1.36	1.29
103N AAT	(1%)	1.28	1.27	1.16	1.16	1.38	1.41	1.30	1.18
103K AAG	(0%)	0.13	0.06	0.12	0.006	0.15	0.04	0.05	0.009
C. Plasmids with 90% wt (AAA), 5% 103N (AAC) and 5% 103N (AAT) at position 103. Results of ASPCR using polymorphism specific primers
Allele	Percent	I	II	III	IV	V	VI	VII	VIII
103K AAA	(90%)	88.77	89.46	88.90	89.82	88.23	88.13	88.82	89.27
103N AAC	(5%)	5.00	5.33	5.26	5.20	5.71	5.66	5.71	5.29
103N AAT	(5%)	6.03	5.11	5.58	4.97	5.75	6.15	5.34	5.42
103K AAG	(0%)	0.21	0.11	0.27	0.02	0.31	0.06	0.12	0.02