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. 2010 Aug 3;4:44. doi: 10.3389/fnbeh.2010.00044

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Copyright © 2010 Parsons, Gafford, Helmstetter.

This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.

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Opioid receptor blockade does not affect ERK phosphorylation following four trials of extinction. (A) Schematic showing the behavioral procedures employed in this experiment. (B) Freezing during the four trials of extinction training. (C) Representative image taken using Nomarski optics at 10×. Arrow indicates the approximate infusion site in the vlPAG. Expression of phosphorylated ERK in the amygdala (D) and mPFC (E) for rats infused with saline (N = 6, black) or naloxone (N = 6, red) and given four trials of extinction training.