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. Author manuscript; available in PMC: 2010 Aug 24.
Published in final edited form as: J Immunol. 2007 Sep 15;179(6):3792–3803. doi: 10.4049/jimmunol.179.6.3792

Figure 6. Ectopic IFNAR2 expression promotes sustained STAT4 phosphorylation and IFN-γ secretion in response to IFN-α.

Figure 6

(A) Spleen and lymph node cells from DO11.10 mice were activated with OVA peptide under Th1-inducing conditions and transduced with retrovirus vectors expressing GFP alone (GFPRV) or the full-length mIFNAR2 subunit. Cells were sorted on day 7 based on GFP expression and restimulated with irradiated BALB/c splenocytes and OVA peptide. Following expansion for an additional 7 days, resting cells were activated with either IL-12 or IFN-α for the times indicated in the figure. Cells were then stained and analyzed for intracellular tyrosine-phosphorylated STAT4 as described in Fig. 5. (B and C) Day 14 transduced Th1 cells were activated with either IL-12 + IL-18, IFN-α + IL-18, or with the individual cytokines indicated in the figure for 24 hours. Brefelden A was added during the last 4 hours of stimulation. Cells were then stained for mCD4 and IFN-γ and analyzed by flow cytometry. Data were gated on live cells and GFP expression.