Table 1. PCR primers used in this study.
Primer | Forward (5'-3') | Reverse (5'-3') | Tm (°C) | Product size (bp) |
---|---|---|---|---|
exon1 |
CTCTGTCTTCCCCCATGAAG |
AGCAGGTCACTACGAGCCATA |
62 |
785 |
exon2 |
TAGTCAATCCTTGGGCCATT |
ACCACGTGGGCACAAAAG |
60 |
561 |
exon3-1 |
CTTCCGCATGATCATTGT |
CTTCCGCATGATCATTGT |
58 |
352 |
exon3-2 |
ATACTGCCTAGGCCACTGGAA |
CCGCTATAAGTACAGCAGCATGAT |
58 |
440 |
exon3-3 | GCCTTCATCATCTGTGGCAC | CAGGCAGCTTTGACTGCTTT | 58 | 342 |