. 2001 Feb 13;98(4):1432–1436. doi: 10.1073/pnas.98.4.1432

Table 2.

The binding site sequences used in gel-shift experiments with the TFIIIA-ZIF fusion peptides and the binding affinities obtained

Binding site name	Binding site sequence^*	Apparent K_d, pM
Binding site name	Binding site sequence^*	TF(1–4)-ZIF	TF-flex-ZIF
ZIF	`GCGTGGGCG`	2,000	1,800
TF5Z	`GCGTGGGCGX₅GGATGGGAGAC`	21	63
TF6Z	`GCGTGGGCGX₆GGATGGGAGAC`	17	68
TF7Z	`GCGTGGGCGX₇GGATGGGAGAC`	3	57
TF8Z	`GCGTGGGCGX₈GGATGGGAGAC`	3	61
TF9Z	`GCGTGGGCGX₉GGATGGGAGAC`	15	58

Nonbound DNA bases in the target sequence are shown by a boldface X. The exact base composition of these gaps was found to have no significant effect on peptide affinity.