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. 2010 Aug 24;5(8):e12315. doi: 10.1371/journal.pone.0012315

Figure 3. Math5 embryonic eye RT-PCRs with increasing amounts of betaine.

Figure 3

A. Agarose gel showing cDNA products amplified from DNase-treated E14.5 eye RNA with UTR primers LP8 and LP4 in the presence of 0X, 1X, 2X and 3X Masteramp™. When the betaine concentration was increased, only the full-length 1087 bp Math5 cDNA product was visible; the 448 bp ECO product was absent. No amplimers were observed in the absence (−) of RNA template or RT enzyme. The identity of all PCR products was verified by sequencing. B. Similar PCR with a mouse genomic DNA template, showing amplification of the identical full-length 1087 bp product. C,D. Parallel PCRs were performed using internal primers LP6 and LP7. A single 486 bp Math5 product was amplified from cDNA or gDNA in 2–3X Masteramp™.