Figure 6. Bmi1-deficiency does not greatly affect the binding of Zfp277 to the Ink4a/Arf locus in early passage presenescent cells.

A–G. The ChIP analysis of the p19^Arf exon 1b and p16^Ink4a exon 1a in Bmi1 ^−/− MEFs. Wild-type and Bmi1 ^−/− MEFs (P-2) pretreated with 10 mM NAC were subjected to ChIP analyses using the indicated antibodies. Percentages of input DNA are shown as the mean ± S.D. for multiple experiments (n>5). Statistical significance was determined with Student's t-test; *p<0.05, **p<0.01, ***p<0.001.