Table 1. PCR primers and conditions.

Set	Forward primer	Reverse primer	Gene region	Annealing temperature (°C)	Product size (bp)
FOXE3 primers [3]
1	tgtccatataaagcgggtcg	atgtacgagtagggcggctt	Exon 1 (N-terminus)	56	298
2	ttctctggcttccctgccct	tcggtgatgaagcggtagat	Exon 1 (N-terminus, forkhead domain)	57	271
3	aagccgccctactcgtacat	tcgttgagcgtgagattgtg	Exon 1 (Forkhead domain)	56	170
4	ttcatcaccgaacgctttgc	aggaagctgccgttgtcgaa	Exon 1 (Forkhead domain)	56	185
5	aagggcaactactggacgct	tagctccggctgcaggttca	Exon 1 (Forkhead domain, C-terminus)	55	267
6	tctgttcagcgtcgacagc	acaggtcgcacaggtgcct	Exon 1 (C-terminus)	55	352
PAX6 primers [37]
1	ctcatttcccgctctggttc	aagagtgtgggtgaggaagt	Exon 1	60	197
2	ttatctctcactctccagcc	aagcgagaagaaagaagcgg	Exon 2	60	276
3	tcagagagcccatcgacgtat	ctgtttgtgggttttgagcc	Exon 3	60	193
4	ttgggagttcaggcctacct	gaagtcccagaaagaccaga	Exon 4	60	153
5	cctcttcactctgctctctt	atgaagagagggcgttgaga	Exon 5	60	257
5a	tgaaagtatcatcatatttgtag	gggaagtggacagaaaacca	Exon 5a	55	237
6	gtggttttctgtccacttcc	aggagagagcattgggctta	Exon 6	60	299
7	caggagacactaccatttgg	atgcacatatggagagctgc	Exon 7	60	252
8	gggaatgttttggtgaggct	caaagggccctggctaaatt	Exon 8	60	371
9	gtagttctggcacaatatgg	gtactctgtacaagcacctc	Exon 9	60	206
10	gtagacacagtgctaacctg	cccggagcaaacaggtttaa	Exon 10	60	243
11	ttaaacctgtttgctccggg	ttatgcaggccaccaccagc	Exon 11	60	208
12	gctgtgtgatgtgttcctca	tgcagcctgcagaaacagtg	Exon 12	60	227
13	catgtctgtttctcaaaggga	gaacaattaacttttgctggcc	Exon 13	55	957
PITX2 primers [24]
1a	ttggctcctaagtgcccc	ccagactcgcattatctcac	Exon 1a	56	596
1b	cttgacacttctctgtcagg	aagcgggaatgtctgcagg	Exon 1b	56	667
2	tagtctcatctgagccctgc	cactggcgatttggttctga	Exon 2	56	282
3	acgcctctctccgcacgt	ttcttgcgctttcgcccga	Exon 3	56	258
4	cagctcttccacggcttct	ttctctcctggtctacttgg	Exon 4	56	374
5a	gtaatctgcactgtggcatc	ctgtgggtgcggctcaca	Exon 5	56	627
5b	ctgagactgaaagcaaagca	ctcccatgaaataaaacacattt	Exon 5	56	787
PITX3 primers [31]
1	cctggtctgccataaagtga	attctcgacctgttcccaag	Exon 1	55	343
2	acgcagccccagctttac	aagccagcgcatattctcc	Exon 2	55	395
3	gtgcaggacataacagcttc	gagcagaggctggaggttg	Exon 3	55	528
4a	ctctagccacctcatctcg	aggcataagggcaggacac	Exon 5	55	524
4b	agacctttccattcgccttc	agtcaaaatgaccccagtcc	Exon 5	55	483