Table 2.

The binding site sequences used in gel-shift experiments with the 3 × 2F ZMS and 2 × 3F ZM peptides and the binding affinities obtained

Binding site name	Binding site sequence	Apparent Kd, pM
		3 × 2F ZMS	2 × 3F ZM
ZIF	GCG TGG GCG	1.1 × 105	2,200
123456	GCG GAC GCG GCG TGG GCG	0.6	1.4
123///56	GCG GAC ATC GCG TGG GCG	890	15
123//56	GCG GAC TC GCG TGG GCG	270	14
123/56	GCG GAC T GCG TGG GCG	630	14
12356	GCG GAC GCG TGG GCG	2.2 × 104	360

Binding site residues that were mutated (and subsequently deleted) are underlined.