Table 3.

The binding site sequences used in gel-shift experiments with the 3 × 2F peptides and the binding affinities determined

Binding site name	Binding site sequence*	Apparent Kd, pM
		3 × 2F ZMS	3 × 2F ZML	3 × 2F ZMSL	3 × 2F ZMLS
123456	GCG GAC GCG GCG TGG GCG	0.6	0.9	ND	ND
12/34/56	GCG GAC T GCG GCG T TGG GCG	1.8 × 104	5	110	120
12//34//56	GCG GAC TC GCG GCG TC TGG GCG	ND	1.1 × 104	1.2 × 104	1.2 × 104
1234/56	GCG GAC T GCG GCG TGG GCG	54	ND	3	89
12/3456	GCG GAC GCG GCG T TGG GCG	77	ND	73	5

ND, not done, represents experiments for which K_ds were not calculated. 

^*Designed gaps in the target sequence are shown in bold.