Table 1.
Product | Type of cultivation | Induction | OD600 at induction | μ before induction (h-1) | μ after induction (h-1) | final OD600 |
---|---|---|---|---|---|---|
AcrA-O1 | LB batch bioreactor | ara + IPTG | 0.5 | 1.15 | 0.12 | 0.9 |
AcrA-O1 | LB batch bioreactor | IPTG | 0.5 | 1.12 | 0.23 | 1.3 |
AcrA-O1 | LB batch shake flask | ara + IPTG | 0.5 | 1.08 | 0.15 | 1.5-2.0 |
AcrA-O1 | LB batch shake flask | ara | 0.5 | 0.86 | 0.56 | 2.2 |
AcrA-O1 | Fed-batch strategy A | ara + IPTG | 47 | 0.24 | 0.08 | 58 |
AcrA-O1 | Fed-batch strategy B | ara + IPTG | 14 | 0.55 | 0.16 | 59 |
AcrA-O1 | Fed-batch strategy C | ara + IPTG | 31 | 0.30 | 0.13 | 42 |
EPA-O1 | LB batch shake flask | ara + IPTG | 0.5 | 0.92 | 0.18 | 1.9 |
EPA-O1 | Fed-batch strategy C | ara + IPTG | 35 | 0.60 | 0.12 | 78 |