Figure 1. Reaction schemes under consideration.

(a) Schematic diagram of the transitions between various states of enzyme for an arbitrary reaction scheme discussed in Methods. The detailed balance condition is shown for the loop comprised by states 1—4. For simplicity only reversible transitions are shown and catalytic transitions are omitted. The rate constants for ligand-binding reactions are proportional to concentrations as indicated. (b) Reaction scheme for random order bisubstrate reaction. (c) Reaction scheme for partial noncompetitive inhibition.