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. 2010 Aug 24;8(8):e1000465. doi: 10.1371/journal.pbio.1000465

Figure 7. The central anti-inflammatory response mediated by exercise requires augmented hypothalamic levels of IL-6 and IL-10.

Figure 7

Western blots showing hypothalamic lysates from C3H/NeN and C3H/HeJ mice under resting conditions or after physical activity; (A) IL-6 and (B) IL-10 expression. Anorexigenic effects of insulin (C) or leptin (D) in C3H/NeN and C3H/HeJ mice under resting conditions, after thapsigargin, thapsigargin plus exercise, and thapsigargin plus recombinant IL-6 or IL-10. Western blots showing hypothalamic lysates from mice; (E) IL-10 expression at 2 h after intrahypothalamic injection of recombinant IL-6 (200 ng) in C3H/NeN and C3H/HeJ mice under resting conditions. (F) IKKβ, (G) PERK, and (H) IRS-1Ser307 phosphorylation and (I) Insulin-induced Akt serine phosphorylation and (J) leptin-induced STAT3 tyrosine phosphorylation in the hypothalamus of C3H/HeJ mice after intrahypothalamic infusion of DMSO, thapsigargin, thapsigargin plus exercise, and thapsigargin plus recombinant IL-6 or IL-10. Data are the means ± SEM. ** p<0.05 versus respective control group at rest; # p<0.05 versus respective control group non-stimulated or stimulated with DMSO; * p<0.05 versus thapsigargin; n = 5–6 animals per group. C3H/NeN (yellow bars) and C3H/HeJ (blue bars). (K) Co-localization of IL-10R (red) with NPY, AgRP, and POMC (green) was evaluated using in situ hybridization technique in the hypothalamus of lean rats, with 20× and 63× magnification. (L) Co-localization of IL-6R (green) and IL-10R (red) in the arcuate nuclei of lean rats, with 200× magnification (scale bar, 10 µm).