Figure 6. Matched and mismatched ligation junctions.

A) Minimal ligation junction with Watson-Crick paired tethered mononucleotide using the A₂Y or T₂Y bridge. B) Single nucleotide mismatch in the minimal ligation junction such as those in lanes 3–5 of Figure 4A, C) Single nucleotide mismatch on the upstream side of the ligation junction (not efficiently ligated by T4 DNA ligase), and D) Up to 5 nt DNA mismatches on the downstream side of the ligation junction (ligated at moderate efficiency by T4 DNA ligase at low ATP concentrations).