Table 1. Effect of spacer length in the DNA bridge on the ligation efficiency.^a .

	Yield (%)
	2h		6h		20h
BRIDGE OLIGO	LIGATION	ADENYLATE	LIGATION	ADENYLATE	LIGATION	ADENYLATE
A0G	0.4	12	5.8	17	19	9.9
A1G	15	36	69	12	87	3.6
A2G	31	37	84	7.3	91	2.9
A3G	11	14	39	7.6	63	6.8
A4G	2.8	13	20	11	48	4.6
A6G	0.7	6.7	1.4	8.5	9.1	10
A8G	0.0	6.0	0.0	6.6	0.8	6.8
A10G	0.0	5.9	0.0	6.9	0.1	11
T1G	9.9	66	51	41	87	6.0
T2G	86	8.5	93	3.7	94	3.2
T3G	31	32	84	7.0	92	2.6
T4G	20	48	79	14	92	2.7
T6G	10	44	59	24	88	3.6
T8G	1.6	21	8.1	37	43	20
T10G	0.0	10	0.6	19	6.0	30
HEG-G	11	37	42	35	69	29

^aReactions using dCHr8 were performed at 20°C using 3.3 µM [³²P]-labeled dCHr8, 4.1 µM of capture oligo and 4.1 µM of the indicated bridging template. All bridging oligos contained dG in the pairing position across from the tethered dC.