Table 2. Simulated Multiplex Ligations: Mock1.a .
2 h | 4h | 6h | 8h | 20h | ||||||
LIG | ADEN | LIG | ADEN | LIG | ADEN | LIG | ADEN | LIG | ADEN | |
32P-dCHr8 | 5 | 36 | 14 | 41 | 26 | 35 | 40 | 31 | 76 | 9 |
32P-dTHr8 | 0.03 | 13 | 0.16 | 17 | 0.37 | 21 | 1 | 26 | 6 | 36 |
32P-dAHr8 | 0.07 | 22 | 0.45 | 35 | 1 | 45 | 2 | 52 | 14 | 53 |
32P-dGHr8 | 5 | 74 | 12 | 77 | 18 | 69 | 28 | 60 | 61 | 23 |
32P-dIHr8 | 0.36 | 35 | 2 | 49 | 4 | 57 | 7 | 60 | 26 | 46 |
Capture oligo and complementary bridging template were in slight excess of the radiolabeled strand. The species that carried the radiolabel is indicated in the first column.