Table 3. Simulated Multiplex Ligations: Mock2.a .
2 h | 4h | 6h | 8h | 20h | ||||||
LIG | ADEN | LIG | ADEN | LIG | ADEN | LIG | ADEN | LIG | ADEN | |
32P-dCHr8 | 9 | 19 | 16 | 13 | 22 | 10 | 28 | 9 | 35 | 6 |
32P-dTHr8 | 0.25 | 13 | 1 | 16 | 2 | 16 | 2 | 18 | 7 | 15 |
32P-dAHr8 | 1 | 34 | 3 | 41 | 6 | 41 | 10 | 38 | 20 | 27 |
32P-dGHr8 | 32 | 53 | 57 | 31 | 71 | 18 | 80 | 10 | 90 | 4 |
32P-dIHr8 | 5 | 50 | 12 | 51 | 20 | 46 | 29 | 38 | 55 | 14 |
Capture oligo and complementary bridging oligo were in slight excess of all dXHr8 species present in the reaction.
The species that carried the radiolabel is indicated in the first column.