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Effects of amino acid substitutions of F122 on binding specificities. ALG-2-interacting proteins in HEK293 cells were pulled down with GST-fused ALG-2 of wild type (WT), ALG-2ΔGF122 (ΔGF122), and F122-substituted mutants. Unfused GST was used as a negative control (Ctrl). Proteins bound to glutathione Sepharose beads were subjected to Western blotting with antibodies against Alix, TSG101, Sec31A, annexin A7, and annexin A11.
