Figure 5.

Augmentation of staurosporine-induced cell death by ALG-2^F122A. Parental (Prnt) HeLa cells and ALG-2-knockdown (ALG-2_KD) HeLa cells were transfected with a vector (pcDNA3) as controls. ALG-2_KD cells were transfected with plasmids that express wild-type ALG-2, ALG-2^ΔGF122 or ALG-2^F122A and cultured for 24 h. The transfected cells were treated with 1 μM staurosporine for 24 h. Cell death was monitored by measuring the amounts of lactate dehydrogenase (LDH) released into the culture medium as described in Materials and Methods. Total amounts of LDH released into the medium and retained in cells were expressed as 100%. Duplicate assays of five independent repetitive experiments were performed and data are presented as means +/- SD (n = 5). Closed column, not treated with staurosporine; open column, treated with staurosporine. Statistically significant differences among staurosporine-treated cells were evaluated by Student's t-test by comparing the measured values of samples with those of control ALG-2_KD cells that were transfected with a control vector (*, P < 0.05; **, P < 0.01)