Table 3.
Loss of intramolecular interactions in F122A mutant
| WT | F122A | ||||
| interacting atom | MFa | Cab | Znc | Znd | |
| Distance (Å) | |||||
| F122 | A122 | ||||
| hydrogen | |||||
| N | OA118 | ND | 2.9 | 3.2 | NDe |
| OL119 | 3.0 | 3.1 | ND | 3.2 | |
| hydrophobic | |||||
| CB | CD1Y124 | 3.7 | 3.6 | 3.9 | ND |
| CE1Y124 | 3.8 | 3.8 | 4.0 | ND | |
| CD1 | CA118 | ND | ND | 4.0 | -f |
| CAL119 | 3.9 | ND | 4.0 | - | |
| CD2L119 | 3.9 | 3.7 | 4.0 | - | |
| CE1Y124 | 3.9 | ND | ND | - | |
| CE1 | CH2W95 | 3.9 | ND | ND | - |
| CZ3W95 | 3.9 | ND | ND | - | |
| CE2 | CG1V98 | ND | 4.0 | ND | - |
| CZ | CH2W95 | 3.9 | ND | ND | - |
| CZ3W95 | 3.5 | ND | 3.9 | - | |
| CG1V98 | 3.8 | 3.5 | 3.6 | - | |
a Metal-free form of des3-20ALG-2
b Ca2+-bound form of des3-20ALG-2
c Zn2+-bound form of ALG-2
d Zn2+-bound form of des3-20ALG-2F122A
e Not detected in the range of 2.6-3.2 Å for hydrogen bonding and 3.2-4.0 Å for hydrophobic interaction
f Not present in des3-20ALG-2F122A