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. 2010 Aug 6;10:25. doi: 10.1186/1472-6807-10-25

Table 4.

Angles and distances calculated for ALG-2 EF3. ALG-2 EF3 has α4 (entering helix, residue No. 93-102) and α5 (exiting helix, residue No. 112-122), but a segment of α5 (residue No. 112-120, common to ALG-2ΔGF122) was used.

Angle (°) Distance (Å)

θ φ ω inner ends midpoint outer ends
WT MFa 49.5 104 137 10.6 12.2 14.7
Cab 57.6 96.9 131 10.7 12.7 15.6
Znc 54.5 98.0 130 10.7 12.6 15.4
ΔGF122 Cad 58.6 88.8 134 10.7 13.1 16.3
F122A Zne 62.6 97.6 128 11.0 13.2 16.4

a Metal-free form of des3-20ALG-2

b Ca2+-bound form of des3-20ALG-2

c Zn2+-bound form of ALG-2

d Ca2+-bound form of des3-23ALG-2ΔGF122

e Zn2+-bound form of des3-20ALG-2F122A

Angles and distances were estimated by the Vector Geometry Mapping (VGM) software (Ref. 50)