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. 2001 Feb 6;98(4):1448–1453. doi: 10.1073/pnas.041329498

Figure 2.

Figure 2

BN/PAGE of metabolically and surface-labeled GlyTs. (Left) Oocytes injected with 25 ng of the indicated cRNAs were labeled metabolically with [35S]methionine or surface-labeled with [125I]sulfo-SHPP, respectively. After solubilization with 1% (wt/vol) digitonin, the His6-tagged GlyT proteins were isolated by Ni2+-NTA chromatography and resolved by 4–13% BN/PAGE. (Right) For direct comparison, the [35S]methionine-labeled and [125I]-labeled GlyT1-His preparations were separated on adjacent lanes of a native 4–13% polyacrylamide gradient gel.