Figure 3.

SDS/PAGE of metabolically (Left) and surface-labeled (Right) GlyTs before and after deglycosylation. Affinity-purified GlyT proteins were denatured in reducing SDS sample buffer, incubated for 2 h or 24 h, at 37°C with the enzymes indicated (5 units of endo H, 2.5 units of PNGase F), and analyzed on an 8% polyacrylamide gel. Different deglycosylation intermediates of GlyT1 and its fully deglycosylated form are indicated by 1–3 and 0, respectively. * and †, complex-glycosylated and core-glycosylated GlyT polypeptides.