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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Dev Biol. 2010 Jul 1;345(2):248–255. doi: 10.1016/j.ydbio.2010.06.026

Fig. 1.

Fig. 1

Purification and identification of the SxlPe and zen VRE-binding factor Grainyhead. (A) Purification scheme used to identify factor(s) binding to SxlPe and the zen VRE. (B) DNase I protection by Mono S fractions (indicated above each lane) using a SxlPe DNA fragment. (C) Analysis of proteins eluting from the DNA-affinity column by SDS-PAGE and silver staining (top). The two polypeptides eluting from the column at 0.25 M and 0.5 M KCl were identified as Grh by mass spectrometry. Asterisks denote keratin. 15% of each elution was loaded. IN, 1% of input. FT, 1% of flow through. W, 1.5% of wash. Shown below is an immunoblot using αGrh antibodies on protein from the DNA-affinity column. 5% of each elution was loaded. (D) SDS-PAGE and silver stain analysis of the purified recombinant Grh. Molecular weight (left) is indicated in kD. (E) DNase I protection by increasing amounts of recombinant Grh (rGrh) (left) or purified protein from nuclear extract (right) of a zen VRE DNA fragment. +, protein from embryonic nuclear extract. −, no protein.