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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Cell Microbiol. 2009 Jan 21;11(5):769–779. doi: 10.1111/j.1462-5822.2009.01290.x

Table 1.

Constructsa SCb SC-TDOc
pYpkA(442-733)d-DBD + pRhoA-AD +++ +++
pCP0236(101-279)-DBD + pDEST22 +++ −−
pCP0236(101-279)-DBD + pAct1(1-565)-AD +++ +++
pCP0236(101-279)-DBD + pAct1(306-565)-AD +++ +++
pCP0236(101-279)-DBD + pAct1(361-565)-AD +++ −−
pCP0236(101-279)-DBD + pAct1(301-360)-AD +++ +++
a

Respective constructs were created in the binding domain (DBD) vector pDEST32 or activating domain (AD) vector pDEST22.

b

Growth on vector selective medium lacking leucine and tryptophan.

c

Growth on high stringency selective medium containing 30 mM 3AT and lacking leucine, tryptophan, and histidine.

d

Numbers in parentheses indicate residues of respective proteins comprising the chimeric constructs.