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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Dev Biol. 2010 Jul 13;345(2):156–169. doi: 10.1016/j.ydbio.2010.07.005

Figure 6. 3-D reconstruction of brunol1 overexpression phenotype.

Figure 6

Serial histological sections were taken from control and brunol1 injected NF44 whole guts. Color overlays added to help visualize differences (Blue-pancreas, yellow-gut endoderm, pink-liver). (A–C) Representative sections of control whole guts. (D) 3-D reconstruction of control whole guts using Amira software. Notice that the pancreas does not encircle the duodenum. (E–G) Representative sections of whole guts isolated from tadpoles injected with brunol1 mRNA. (H) 3-D reconstruction reveals increased amounts of pancreatic tissue such that it now encircles the duodenum. Blue-pancreas, yellow-gut endoderm, pink-liver.