Figure 5.—

Serial dilution yeast two-hybrid testing of the effect of the Mcm10^d08029 allele on known protein interactions. The left column shows growth on media lacking histidine and the right column shows growth control on media with histidine. The top panel shows one-hybrid control showing no growth for empty vector controls. The middle panel shows the relative strength of two-hybrid interactions between Mcm10 and the proteins indicated. The bottom panel demonstrates the effect of the Mcm10^d08029 85-aa C-terminal truncation on the relative strength of these same interactions.