Figure 3.—

Localization of Su(var)3-7 in polytene chromosomes from JIL-1, Su(var)3-7, and Su(var)3-9 mutant female third instar larvae. The polytene squashes were labeled with antibody to Su(var)3-7 (in green) and with Hoechst (DNA, in blue/gray). The chromocenter is indicated with an asterisk and n indicates weak background labeling of the nucleolus in some of the preparations. Preparations from wild-type, JIL-1^z2 homozygous (z2/z2), JIL-1^z2 Su(var)3-7¹⁴/JIL-1^z2 (z2, 3-7¹⁴/z2), and Su(var)3-9⁰¹/Su(var)3-9⁰² (3-9⁰¹/3-9⁰²) larvae are shown. In wild-type preparations, Su(var)3-7 labeling was mainly localized to and abundant at the chromocenter; however, in the absence of the JIL-1 kinase, the Su(var)3-7 labeling spread to the chromosome arms with a concomitant decrease at the chromocenter. In JIL-1^z2 Su(var)3-7¹⁴/JIL-1^z2 and Su(var)3-9⁰¹/Su(var)3-9⁰² mutant larvae Su(var)3-7 labeling was greatly reduced and mainly confined to the chromocenter.