Figure 2.—

Molecular cloning and sequence analysis of moc-3 defined by a lin-1(e1275) suppressor. (A) Cartoon of the moc-3 location relative to mapping markers and rescuing DNAs and regions uncovered by deletion mutations. The asterisk indicates the site of ku300 mutation. (B) Alignment of MOC-3 and its orthologs in other species. The orthologs were chosen from the representative eukaryotic species of H. sapiens (MOCS3), Danio rerio (LOC393095), D. melanogaster (CG13090), Arabidopsis thaliana (Cnx5), and S. cerevisiae (UBA4). Alignment of multiple protein sequences was produced by Clustalw (http://align.genome.jp/) and edited manually by using GENEDOC (http://www.nrbsc.org/gfx/genedoc/).