Figure 6.—

A product of translational readthrough of lin-1(e1275) may be observed in the moc-3(ku300) background. (A) Schematic of the pSK002 construct. lin-1(e1275) cDNA was fused to GFP. NLS, nuclear localization signal. (B) The readthrough at R175Opal in lin-1(e1275) likely resulted in the expression of the LIN-1∷GFP full-length protein in the nucleus. Shown here is the head region of representative L4 larvae. A sur-5 promoter-driven DsRed construct was used as a marker for transgenic worms. Full genotypes are Ex[pSK002], unc-119(ed3); him-5(e1467); Ex[pSK002], moc-3(ku300); Ex[pSK002], unc-119(ed3); moc-3(ku300); Ex[pSK002]. Bars, 20 μm.