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Coimmunoprecipitation–Western blot experiments. (A) HDAC1 and HDAC2 were immunoprecipitated from T-Ag Jurkat extracts and Western blotted for CoREST. (B) Polypeptides specific to CoREST and MTA2 were immunoprecipitated from HeLa extract and Western blotted. (C) Polypeptides associated with recombinant p110-FLAG were immunoprecipitated and Western blotted for components of various HDAC complexes.
