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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Gene Ther. 2010 May 20;17(10):1244–1252. doi: 10.1038/gt.2010.62

Figure 3. Hypoxic environment and/or reducing agent improve the transduction efficiency and viability of human Fanconi bone marrow progenitors.

Figure 3

Human Fanconi bone marrow mononuclear cells were transduced overnight with the GFP vector at a vector to cell ratio of 10:1 and plated in colony assays at 20,000 cells per plate. During the transduction and colony assays, cells were exposed to either 21% or 5% O2 with or without 1mM NAC, a reducing agent. Transduction efficiency increased from 16.7% to 47.8% by reducing oxygen from 21% to 5% and adding 1 mM NAC.