Figure 5. There is superior survival of FancA-sW transduced FANCA bone marrow cells in culture in MMC than control GFP vector transduced cells.

Human Fanconi bone marrow mononuclear cells were transduced overnight with FancA-sW (FA) or GFP (GFP=RRL.sin.cPPT.PGK.GFP.wpre) vector at a vector to cell ratio of 10:1. The cells were subsequently plated into 96-well plates and exposed to increasing MMC concentrations in triplicate. Viable cells were counted at 48 and 96 hours after exposure to MMC. During the transduction and MMC challenge, cells were placed in 5% O₂ with 1 mM NAC. A. Survival cultures for patient 5 (upper) and patient 6 (lower) for transduced cells with the indicated vector at the indicated time of culture, 48 or 96 hours. B. Survival cultures for patient 7, upper curves at the 48 hr time point and lower curves at the 96 hr time point for the indicated vector, as above. Significant differences were determined by student t-test comparing cell survival of FancA-sW to GFP vector transduced bone marrow for the same MMC concentration at the same time point, and the p values are listed in Table 1. Blue lines indicate FancA-sW transduced cells, green lines control GFP vector transduced cells.