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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Biochim Biophys Acta. 2009 Mar;1790(3):198–207. doi: 10.1016/j.bbagen.2009.01.001

Figure 5.

Figure 5

Delivery of DNA (labeled with Alexa647) and chimeric fusion proteins (containing the GFP fluorophore) to PC12 cells as monitored by flow cytometry. The protein amount was fixed in all experiments at 2.8 nmol (a) Uptake with a mixture of GT and 30bp DNA. (b) Uptake with a mixture of GT and 293bp DNA. (c) Uptake with a mixture of PG and 30bp DNA. (d) Uptake with a mixture of PG and 293bp DNA. (e) Uptake with a mixture of PGT and 30bp DNA. (f) Uptake with a mixture of PGT and 293bp DNA. In all experiments, the 30bp DNA fragment contained a single Alexa647 fluorophore, while the 293bp fragments were doubly labeled. Error bars represent standard errors. Each experiment was performed in at least triplicate (n ≥ 3). The * indicates the value is significantly different from the protein-only control (DNA amount = 0) using one-way ANOVA and post-hoc Tukey’s test (p < 0.05).