8,9-EET analog(214) inhibits the caspase-3 activation, the expression of caspase-9 and cleavage of procaspase-3 through ROCK pathway. A: The expression of caspase-9 in rat PASMCs. B: The expression of procaspase-3 in rat PASMCs. C: The activity of Caspase-3 in rat PASMCs. 8,9-EET analog(214) decreased the activity of caspase-3, suppressed the cleavage of procaspase-3 and reduced the expression of caspase-9 in PASMCs under serum deprivation. However, its inhibitory effects were abolished after blocking the ROCK pathway with Y-27632 or HA-1077. “SD” means serum deprivation. All values are denoted as means ± S.E.M. from three or more independent batches of cells (*p<0.05 compared with Control; #p<0.05 compared with SD).