. Author manuscript; available in PMC: 2011 May 7.

Published in final edited form as: Biochem Biophys Res Commun. 2010 Apr 10;395(3):416–419. doi: 10.1016/j.bbrc.2010.04.038

Table 1.

hE3 and PDC specific activities of WT-hE3 and its mutants. WT- and mutant-hE3 activities were measured in their free forms in both the forward and reverse directions. The PDC activities of hE3s were measured after reconstitution with recombinant WT-hE1 and WT-hE2-BP subcomplex.

hE3	hE3 activity(forward reaction) (%)	hE3 activity(reverse reaction) (%)	PDC activity (%)
WT	100	100	100
H348A	60	66	92
H348L	65	74	97
D413A	85	79	18
D413N	119	96	85
Y438A	100	91	9
Y438F	100	112	98
Y438H	99	92	20
R447A	110	122	110

WT-hE3 activity (100%) was 617 ± 47 units/mg (n=5) in the forward direction and 195 ± 23 units/mg (n=5) in the reverse direction. Reconstituted PDC activity with WT-hE3 (100%) was 16.5 ± 1.5 units/mg of hE1 (n=5) [9].