Fig. 4.
RUNX1 and RUNX2 upregulate Gal-3 in HP75 cells and pituitary tumors, promote HP75 cell growth. a siRNA of RUNX1 and RUNX2 knocked down RUNX1 and RUNX2 expression, Gal-3 was knocked down by either of the siRNA. GAPDH served as positive control; non-transfected and non-target siRNA served as negative controls (RT-PCR) in RNA interference experiments. First left lanes are positive controls for PCR reactions. b Densitometry for a. c [3H] Thymidine incorporation experiments showed that knockdown of RUNX1, RUNX2 slowed HP75 cell growth. d Immunohistochemistry analysis with tissue microarray showing correlation of Gal-3, RUNX1, and RUNX2 expression levels in pituitary tumors. Gal-3 was expressed by PRL and ACTH tumors, but not by GH, FSH, LH, null cell, or TSH tumors. In the normal pituitary only PRL, ACTH, and folliculostellate cells expressed Gal-3.
*** P < 0.001; ANOVA test