Table 2.

Probes used in EMSA (the binding motifs are highlighted by bold letters; mutated nucleotides were in lower case)

Probes (i.e., Oligos. In cold probe, there was not biotin labeled)	Sequences
Wild type for region 1
Sense	5′-biotin-TGATGTCTGGTGAGGGCCTGCTTTCTGGTTCACAG
Antisense	5′-biotin-CTGTGAACCAGAAAGCAGGCCCTCACCAGACATCA
Mutant 1 for region 1 site 1
Sense	5′-biotin-TGATGTCTccTGAGGGCCTGCTTTCTGGTTCACAG
Antisense	5′-biotin-CTGTGAACCAGAAAGCAGGCCCTCAggAGACATCA
Mutant 2 for region 1 site 2
Sense	5′-biotin-TGATGTCTGGTGAGGGCCTGCTTTCTccTTCACAG
Antisense	5′-biotin-CTGTGAAggAGAAAGCAGGCCCTCACCAGACATCA
Wild type for region 5
Sense	5′-biotin-GGCTCGGAGCCACCGCCCCGCCGGCGCCC
Antisense	5′-biotin-GGGCGCCGGCGGGGCGGTGGCTCCGAGCC
Mutant type for region 5
Sense	5′-biotin-GGCTCGGAGCCAggGCCCCGCCGGCGCCC
Antisense	5′-biotin-GGGCGCCGGCGGGGCccTGGCTCCGAGCC