Fig. 6.
Aggregation of as-purified ALS-causing SOD1 mutants is caused by their increased propensity to expose hydrophobic surfaces. (a, b) Correlation between surface hydrophobicity measured by Sypro Orange fluorescence and aggregation measured by DLS (a) and ThT fluorescence (b). Data are means and s.e.m. from 3 independent experiments. Measurements were made 20 minutes after TFE treatment. The high linear correlation coefficient R, denotes the strength of the correlation. Note that the MBR mutants are more susceptible to TFE-induced aggregation than the WTL mutants. (c) Aggregation of SOD1G85R and SOD1G37R at room temperature, revealed by DLS.