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. 2010 Jul 8;10:206. doi: 10.1186/1471-2148-10-206

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Copyright ©2010 Biedler and Tu; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Early zygotic promoter activity of AaKLC2.1 upstream sequence in Ae. aegypti embryos. Embryos were injected in triplicate with either one of the following firefly luciferase reporter plasmids: pGL3-basic only (negative control, shown as pGL3) or pGL3-basic containing 1042 bp of AaKLC2.1 5'UTR and upstream sequence. A Renilla luciferase reporter pRL-null containing D. melanogaster actin 5C promoter was used as an injection control. Embryos were homogenized and assayed at 5 hr after deposition. Mean fold relative light units (RLUs) +/- standard error is shown. The mean fold RLU is calculated as the ratio of the firefly luciferase activity to the Renilla luciferase activity. The mean fold activity of AaKLC2.1 was 1604 times greater than pGL3-basic.