Skip to main content
. 2010 Jun 8;59(9):2297–2305. doi: 10.2337/db09-1420

FIG. 1.

FIG. 1.

Analysis of VEGF and HIF-1α expression in normal and diabetic conditional VEGF KO mice. A and B: Immunoblotting analysis for VEGF (A) and HIF-1α (B) in retinas from conditional VEGF KO mice and WT controls 2 months after diabetes. C and D: Confocal microscopic analysis of immunostained retinas for VEGF expression (green) in conditional VEGF KO mice and WT controls subjected to a diabetic stress. Blue: nuclear staining (DAPI). Scale bar equals 40 μm. ONL, outer nuclear layer; INL, inner nuclear layer. Error bar: SEM. ***P < 0.001. ns, not significant. VEGF expression was significantly reduced in the retinas of conditional VEGF KO mice under normal or diabetic conditions. Although diabetes upregulated HIF-1α, no significant change in the levels of retinal HIF-1α was observed in diabetic conditional VEGF KO mice. (A high-quality digital representation of this figure is available in the online issue.)