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. 2010 Jun 8;59(9):2297–2305. doi: 10.2337/db09-1420

FIG. 4.

FIG. 4.

Analysis of tight junction protein expression in retinas of conditional VEGF KO mice 6 months after diabetes. A and B: Immunoblotting and quantification of tight junction protein occludin and ZO-1 in retinas of diabetic conditional VEGF KO mice. Error bar: SEM. ***P < 0.001. C–F: Confocal microscopic analysis of immunostained tight junction protein ZO-1 in retinal vasculature (C and D) from the diabetic retinas of conditional VEGF KO mice. Scale bars represent 50 μm. Loss of Müller cell-derived VEGF caused a significant attenuation of diabetes-induced depletion of tight junction proteins occludin and ZO-1. ns, not significant.